Related Product Information for EpCAM cell lysate
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
This Rat EpCAM overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EpCAM protein from the overexpression lysate was verified.
References
Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
Uniprot Description
Function: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E
By similarity.
Subunit structure: Monomer
By similarity. Interacts with phosphorylated CLDN7. Ref.4
Subcellular location: Lateral cell membrane; Single-pass type I membrane protein. Cell junction › tight junction. Note: Co-localizes with CLDN7 at the lateral cell membrane and tight junction. Ref.4
Post-translational modification: Glycosylation at Asn-198 is crucial for protein stability
By similarity.
Sequence similarities: Belongs to the EPCAM family.Contains 1 thyroglobulin type-1 domain.
Product Notes
The EpCAM epcam (Catalog #
AAA8117543) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 254aa. AAA Biotech's EpCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the EpCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr26 6.
It is sometimes possible for the material contained within the vial of
"EpCAM, Cell Lysate" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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