Human Sodium/Hydrogen Exchanger 3 (NHE3) RTU ELISA Kit | NHE3 rtu elisa kit

Human Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit

Cat. Num. AAA4503579

• Gene Names: SLC9A3; NHE3; DIAR8; NHE-3
• Reactivity: Human
• Synonyms: Sodium/Hydrogen Exchanger 3 (NHE3); Human Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit; SLC9A3; Solute Carrier Family 9 Member 3; Sodium-hydrogen antiporter 3; Na(+)/H(+) exchanger 3; NHE3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 825

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.093ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NHE3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NHE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NHE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NHE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NHE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 547235625
• NCBI GeneID: 6550
• NCBI Accession #: NP_001271280.1
• NCBI GenBank Nucleotide #: NM_001284351.2
• UniProt Accession #: P48764; Q3MIW3; B7ZKR2; E9PF67
• Molecular Weight: 91,904 Da
• NCBI Official Full Name: sodium/hydrogen exchanger 3 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 9 member A3
• NCBI Official Symbol: SLC9A3
• NCBI Official Synonym Symbols: NHE3; DIAR8; NHE-3
• NCBI Protein Information: sodium/hydrogen exchanger 3
• UniProt Protein Name: Sodium/hydrogen exchanger 3
• UniProt Gene Name: SLC9A3
• UniProt Synonym Gene Names: NHE-3

NCBI Description

The protein encoded by this gene is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell. Defects in this gene are a cause of congenital secretory sodium diarrhea. Pseudogenes of this gene exist on chromosomes 10 and 22. [provided by RefSeq, Mar 2016]

Uniprot Description

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient (PubMed:26358773). Plays an important role in signal transduction.