CD8 alpha Active Protein | CD8A active protein

CD8 alpha Protein, Ferret, Recombinant (His Tag)

Cat. Num. AAA8123281

• Purity: >92% as determined by SDS-PAGE
• Synonyms: CD8 alpha; CD8 alpha Protein; Ferret; Recombinant (His Tag); Ferret CD8A/CD8 alpha chain Protein (His Tag); CD8a molecule; CD8A active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu186

• Species: Ferret
• Activity: 1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA.; 2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA.; 3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the Ferret (Mustela putorius furo) CD8A (ABS50091.1) extracellular domain (Met 1-Glu 186) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD8A active protein

Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

References

References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant Ferret CD8A consists of 175 amino acids and has a calculated molecular mass of 19.5kDa. The apparent molecular mass of the recombinant protein is approximately 28-33kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Family: T-cell surface glycoprotein