Goat FUBP1 Polyclonal Antibody | anti-FUBP1 antibody

Anti-FUBP1 Antibody (aa160-174)

Cat. Num. AAA2400883

• Gene Names: FUBP1; FBP; FUBP; hDH V
• Reactivity: Human; Predicted: Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Hamster, Horse, Bat, Monkey
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: FUBP1; Polyclonal Antibody; Anti-FUBP1 Antibody (aa160-174); Rabbit Polyclonal (IgG) to Human FUBP1; DNA helicase V; FBP; HDH V; FUSE-binding protein 1; FUBP; anti-FUBP1 antibody

• Host: Goat
• Reactivity: Human; Predicted: Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Hamster, Horse, Bat, Monkey
• Clonality: Polyclonal
• Specificity: Human FUBP1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 644

• Applicable Applications for anti-FUBP1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:128000; IHC-P: 5 ug/ml; WB: 0.03-0.1 ug/ml
• Immunogen: Synthetic peptide C-DQIVEKGRPAPGFHH from an internal region of human FUBP1 (NP_476513.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Horse, Rabbit, Porcine, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Lizard, Xenopus (93%); Medaka (80%).
• Conjugation: Unconjugated
• Epitope: aa160-174
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-FUBP1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(FUBP1 antibody (0.03 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-FUBP1 antibody

FUBP1 is a single stranded DNA-binding protein that binds to multipleDNA elements, including the far upstream element (FUSE) located upstreamof c-myc. Binding to FUSE occurs on the non-coding strand, and isimportant to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, aDNA-binding central domain, and a C-terminal transactivation domainthat contains three tyrosine-rich motifs.

NCBI and Uniprot Product Information

• NCBI GI #: 17402900
• NCBI GeneID: 8880
• NCBI Accession #: NP_003893.2
• NCBI GenBank Nucleotide #: NM_003902.4
• UniProt Accession #: Q96AE4; Q12828
• Molecular Weight: 68,605 Da
• NCBI Official Full Name: far upstream element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: far upstream element binding protein 1
• NCBI Official Symbol: FUBP1
• NCBI Official Synonym Symbols: FBP; FUBP; hDH V
• NCBI Protein Information: far upstream element-binding protein 1
• UniProt Protein Name: Far upstream element-binding protein 1
• Protein Family: Far upstream element-binding protein
• UniProt Gene Name: FUBP1
• UniProt Synonym Gene Names: FBP; FUSE-binding protein 1; hDH V

NCBI Description

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.