CD8 alpha Recombinant Protein | CD8A recombinant protein

CD8 alpha Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123417

• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD8 alpha; CD8 alpha Protein; Rat; Recombinant (hFc Tag); Rat CD8A/Lyt2 Protein (Fc Tag); CD8a molecule; CD8A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr189

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat CD8A (P07725)(Met1-Tyr189) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD8A recombinant protein

Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

References

References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

NCBI and Uniprot Product Information

• NCBI GI #: 13928726
• NCBI GeneID: 24930
• NCBI Accession #: NP_113726.1
• NCBI GenBank Nucleotide #: NM_031538.2
• UniProt Accession #: P07725
• Molecular Weight: 26,196 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: Cd8a
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; CD8 antigen alpha-chain; CD8 antigen, alpha chain; CD8 antigen, alpha-chain; OX-8 membrane antigen
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd8a
• UniProt Entry Name: CD8A_RAT

Uniprot Description

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.