I-309 Cell Lysate | CCL1 cell lysate
Mouse I-309 HEK293 Overexpression Lysate
Gene Names
Ccl1; I-309; Scya1; Tca-3; BF534335
Applications
Western Blot
Synonyms
I-309; Mouse I-309 HEK293 Overexpression Lysate; Mouse I-309/CCL1/TCA-3 HEK293 Cell Lysate (WB positive control); chemokine (C-C motif) ligand 1; Mouse BF534335 Overexpression Lysate; Mouse I-309 Overexpression Lysate; Mouse Scya1 Overexpression Lysate; Mouse Tca-3 Overexpression Lysate; CCL1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
330aa
Sequence
Lys24-Cys92
Applicable Applications for CCL1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the secreted form of mouse CCL1 isoform 1 (P10146-1) (Lys 24-Cys 92) was fused with the Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CCL1 cell lysate
CCL1 or chemokine (C-C motif) ligand 1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. The C-C chemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands (CCL)-1 to 28. I-309/CCL1/TCA-3 interacts with the G protein-linked transmembrane chemokine receptors CCR8 and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that I-309/CCL1/TCA-3 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, I-309/CCL1/TCA-3 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. I-309/CCL1/TCA-3 induces significant levels of LTC4 from elicited eosinophils.
This Mouse I-309 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of I-309 protein from the overexpression lysate was verified.
This Mouse I-309 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of I-309 protein from the overexpression lysate was verified.
References
Miller MD, et al. (1992) The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci. 89 (7): 2950-4.Roos RS, et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J Biol Chem. 272 (28): 17251-4.Oliveira SH, et al. (2002) Increased responsiveness of murine eosinophils to MIP-1beta (CCL4) and TCA-3 (CCL1) is mediated by their specific receptors, CCR5 and CCR8. J Leukoc Biol. 71(6): 1019-25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
9,836 Da
NCBI Official Full Name
C-C motif chemokine 1
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 1
NCBI Official Symbol
Ccl1
NCBI Official Synonym Symbols
I-309; Scya1; Tca-3; BF534335
NCBI Protein Information
C-C motif chemokine 1; P500; TCA3; CCR8 ligand; SIS-epsilon; T-cell activation protein 3; small inducible cytokine A1; small-inducible cytokine A1
UniProt Protein Name
C-C motif chemokine 1
Protein Family
UniProt Gene Name
Ccl1
UniProt Synonym Gene Names
Scya1; Tca3; TCA-3; TCA3
UniProt Entry Name
CCL1_MOUSE
Uniprot Description
CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis
Research Articles on CCL1
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Product Notes
The CCL1 ccl1 (Catalog #AAA8116442) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 330aa. AAA Biotech's I-309 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CCL1 ccl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Lys24-Cys9 2. It is sometimes possible for the material contained within the vial of "I-309, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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