I-309 Recombinant Protein | CCL1 recombinant protein

I-309 Protein, Human, Recombinant

Cat. Num. AAA8119973

• Gene Names: CCL1; P500; SISe; TCA3; I-309; SCYA1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: I-309; I-309 Protein; Human; Recombinant; Human I-309/CCL1/TCA-3 Protein; P500 Protein; SCYA1 Protein; SISe Protein; TCA3 Protein; chemokine (C-C motif) ligand 1; CCL1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 40% acetonitrile, 0.1% TFA, 20% H2O. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys24-Lys96

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the human CCL1 (P22362)(Lys24-Lys96) was expressed, with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL1 recombinant protein

Background: CCL1 or chemokine (C-C motif) ligand 1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. The C-C chemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands (CCL)-1 to 28. I-309/CCL1/TCA-3 interacts with the G protein-linked transmembrane chemokine receptors CCR8 and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that I-309/CCL1/TCA-3 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, I-309/CCL1/TCA-3 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. I-309/CCL1/TCA-3 induces significant levels of LTC4 from elicited eosinophils.

References

Miller MD, et al. (1992) The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci. 89 (7): 2950-4.Roos RS, et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J Biol Chem. 272 (28): 17251-4.Oliveira SH, et al. (2002) Increased responsiveness of murine eosinophils to MIP-1beta (CCL4) and TCA-3 (CCL1) is mediated by their specific receptors, CCR5 and CCR8. J Leukoc Biol. 71(6): 1019-25.

NCBI and Uniprot Product Information

• NCBI GI #: 4506833
• NCBI GeneID: 6346
• NCBI Accession #: NP_002972.1
• NCBI GenBank Nucleotide #: NM_002981.2
• UniProt Accession #: P22362; Q2M309; B2R5G9
• Molecular Weight: 10,992 Da
• NCBI Official Full Name: C-C motif chemokine 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 1
• NCBI Official Symbol: CCL1
• NCBI Official Synonym Symbols: P500; SISe; TCA3; I-309; SCYA1
• NCBI Protein Information: C-C motif chemokine 1; T lymphocyte-secreted protein I-309; inflammatory cytokine I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• UniProt Protein Name: C-C motif chemokine 1
• Protein Family: Cyclin
• UniProt Gene Name: CCL1
• UniProt Synonym Gene Names: SCYA1
• UniProt Entry Name: CCL1_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; viral reproduction; immune response; signal transduction; chemotaxis