Porcine Dihydropyrimidine Dehydrogenase ELISA Kit | DPYD elisa kit

Porcine Dihydropyrimidine Dehydrogenase ELISA Kit

Cat. Num. AAA740801

• Gene Names: DPYD; DHP; DPD; DHPDHASE
• Reactivity: Porcine
• Synonyms: Dihydropyrimidine Dehydrogenase; Porcine Dihydropyrimidine Dehydrogenase ELISA Kit; DPYD elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This DPYD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine DPYD. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPYD elisa kit

Principle of the Assay: DPYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DPYD antibody and an DPYD-HRP conjugate. The assay sample and buffer are incubated together with DPYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DPYD concentration since DPYD from samples and DPYD-HRP conjugate compete for the anti-DPYD antibody binding site. Since the number of sites is limited, as more sites are occupied by DPYD from the sample, fewer sites are left to bind DPYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DPYD concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DPYD elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 693912
• NCBI GeneID: 1806
• Molecular Weight: 18,936 Da
• NCBI Official Full Name: dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: DPYD
• NCBI Official Synonym Symbols: DHP; DPD; DHPDHASE
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• Protein Family: Dihydropyrimidine dehydrogenase
• UniProt Gene Name: DPYD
• UniProt Synonym Gene Names: DHPDHase; DPD
• UniProt Entry Name: DPYD_HUMAN

NCBI Description

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Oxidoreductase; EC 1.3.1.2; Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; 4 iron, 4 sulfur cluster binding; metal ion binding; dihydroorotate dehydrogenase activity; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; uracil catabolic process; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; pyrimidine base catabolic process; thymidine catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency