Rabbit anti-Human SUMF1 Polyclonal Antibody | anti-SUMF1 antibody

SUMF1, CT (SUMF1, FGE, Sulfatase-modifying factor 1, C-alpha-formylglycine-generating enzyme 1)

Cat. Num. AAA6011375

• Gene Names: SUMF1; FGE; UNQ3037; AAPA3037
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SUMF1; Polyclonal Antibody; CT (SUMF1; FGE; Sulfatase-modifying factor 1; C-alpha-formylglycine-generating enzyme 1); Anti -SUMF1; anti-SUMF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SUMF1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: SUMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the C-terminal region of human SUMF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SUMF1 Antibody (C-Term) in Hela cell line lysates (35ug/lane). SUMF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SUMF1 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SUMF1 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SUMF1 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SUMF1 antibody

SUMF1 encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine.

Product Categories/Family for anti-SUMF1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 257470977
• NCBI GeneID: 285362
• NCBI Accession #: NP_001158147.1
• NCBI GenBank Nucleotide #: NM_001164675.1
• UniProt Accession #: Q8NBK3; Q0VAC6; Q0VAC7; Q2NL78; Q53ZE4; Q6UY39; Q96AK5; Q96DK8; B4DXK5; B7XD05; E9PGL0; G5E9B0
• Molecular Weight: 40,556 Da
• NCBI Official Full Name: sulfatase-modifying factor 1 isoform 3
• NCBI Official Synonym Full Names: sulfatase modifying factor 1
• NCBI Official Symbol: SUMF1
• NCBI Official Synonym Symbols: FGE; UNQ3037; AAPA3037
• NCBI Protein Information: sulfatase-modifying factor 1; FGly-generating enzyme; C-alpha-formylglycine-generating enzyme 1
• UniProt Protein Name: Sulfatase-modifying factor 1
• Protein Family: Sulfatase-modifying factor
• UniProt Gene Name: SUMF1
• UniProt Synonym Gene Names: FGE
• UniProt Entry Name: SUMF1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

SUMF1: Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD). MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive. Belongs to the sulfatase-modifying factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Endoplasmic reticulum; Secreted, signal peptide; Oxidoreductase; EC 1.8.99.-

Chromosomal Location of Human Ortholog: 3p26.1

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein homodimerization activity; metal ion binding; oxidoreductase activity

Biological Process: cellular protein metabolic process; sphingolipid metabolic process; glycosphingolipid metabolic process; post-translational protein modification

Disease: Multiple Sulfatase Deficiency