Typical Testing Data/Standard Curve (for reference only)
Sheep Chemokine C-C-Motif Ligand 1 ELISA Kit | CCL1 elisa kit
Sheep Chemokine C-C-Motif Ligand 1 ELISA Kit
Gene Names
Ccl1; I-309; Scya1; Tca-3; BF534335
Reactivity
Sheep
Synonyms
Chemokine C-C-Motif Ligand 1; Sheep Chemokine C-C-Motif Ligand 1 ELISA Kit; CCL1 elisa kit
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL1 elisa kit
Intended Uses: This CCL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CCL1. This ELISA kit for research use only!
Principle of the Assay: CCL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL1 antibody and an CCL1-HRP conjugate. The assay sample and buffer are incubated together with CCL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL1 concentration since CCL1 from samples and CCL1-HRP conjugate compete for the anti-CCL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL1 from the sample, fewer sites are left to bind CCL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CCL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL1 antibody and an CCL1-HRP conjugate. The assay sample and buffer are incubated together with CCL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL1 concentration since CCL1 from samples and CCL1-HRP conjugate compete for the anti-CCL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL1 from the sample, fewer sites are left to bind CCL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CCL1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,836 Da
NCBI Official Full Name
C-C motif chemokine 1
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 1
NCBI Official Symbol
Ccl1
NCBI Official Synonym Symbols
I-309; Scya1; Tca-3; BF534335
NCBI Protein Information
C-C motif chemokine 1; P500; TCA3; CCR8 ligand; SIS-epsilon; T-cell activation protein 3; small inducible cytokine A1; small-inducible cytokine A1
UniProt Protein Name
C-C motif chemokine 1
Protein Family
UniProt Gene Name
Ccl1
UniProt Synonym Gene Names
Scya1; Tca3; TCA-3; TCA3
UniProt Entry Name
CCL1_MOUSE
Uniprot Description
CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis
Research Articles on CCL1
Similar Products
Product Notes
The Sheep CCL1 ccl1 (Catalog #AAA743835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743835 ELISA Kit recognizes Sheep CCL1. It is sometimes possible for the material contained within the vial of "Chemokine C-C-Motif Ligand 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
