Western Blot (WB)
(Western blot detection of BTF3 in C6,3T3 cell lysates using BTF3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)
Rabbit BTF3 Polyclonal Antibody | anti-BTF3 antibody
BTF3 Rabbit pAb
IHC: 1:50
ICC/IF: 1:50
FC/FACS: 1:50
IP: 1:20
Western Blot (WB)
(Western blot detection of BTF3 in C6,3T3 cell lysates using BTF3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)
Western Blot (WB)
(Western blot detection of BTF3 in C6,3T3 cell lysates using BTF3 Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)
When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
Uniprot Description
BTF3: a general transcription factor. BTF3 can form a stable complex with RNA polymerase II. An interaction partner of protein kinase CK2. Required for the initiation of transcription. Expressed at high levels in glioblastoma multiforme. Overexpressed in the microsatellite instability (MIN) phenotypes in colorectal cancer. Up-regulated strongly in the mammary gland during pregnancy, suggesting an involvement in alveolar growth. Two alternatively spliced isoforms have been described.
Protein type: Transcription factor; Transcription initiation complex
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: transcription from RNA polymerase II promoter; protein transport; regulation of transcription, DNA-dependent; in utero embryonic development
Research Articles on BTF3
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Product Notes
The BTF3 btf3 (Catalog #AAA4751841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTF3 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:50 ICC/IF: 1:50 FC/FACS: 1:50 IP: 1:20. Researchers should empirically determine the suitability of the BTF3 btf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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