Rabbit anti-Human XPNPEP2 Polyclonal Antibody | anti-XPNPEP2 antibody

Anti-XPNPEP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105967

• Gene Names: XPNPEP2; APP2; AEACEI
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: XPNPEP2; Polyclonal Antibody; Anti-XPNPEP2 Antibody; Rabbit Polyclonal; XPNPEP2 Antibody; Rabbit PAb; Antigen Affinity Purified; X-prolyl aminopeptidase (aminopeptidase P) 2; membrane-bound; Anti-AEACEI Antibody; Anti-APP2 Antibody; anti-XPNPEP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human XPNPEP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-XPNPEP2 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human XPNPEP2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human XPNPEP2 (rh XPNPEP2). XPNPEP2 specific IgG was purified by Human XPNPEP2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human XPNPEP2 in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of human XPNPEP2 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-XPNPEP2 antibody

Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).

References

Sprinkle TJ, et al. (2000) Cloning, chromosomal sublocalization of the human soluble aminopeptidase P gene (XPNPEP1) to 10q25.3 and conservation of the putative proton shuttle and metal ligand binding sites with XPNPEP2. Arch Biochem Biophys. 378(1): 51-6.Prueitt RL, et al. (2000) Physical mapping of nine Xq translocation breakpoints and identification of XPNPEP2 as a premature ovarian failure candidate gene. Cytogenet Cell Genet. 89(1-2): 44-50.Duan QL, et al. (2005) A variant in XPNPEP2 is associated with angioedema induced by angiotensin I-converting enzyme inhibitors. Am J Hum Genet. 77(4): 617-26.Woodard-Grice AV, et al. (2010) Sex-dependent and race-dependent association of XPNPEP2 C-2399A polymorphism with angiotensin-converting enzyme inhibitor-associated angioedema. Pharmacogenet Genomics. 20(9): 532-6.

NCBI and Uniprot Product Information

• NCBI GI #: 93141226
• NCBI GeneID: 7512
• NCBI Accession #: NP_003390.4
• NCBI GenBank Nucleotide #: NM_003399.5
• UniProt Accession #: O43895; O75994; A0AV16
• Molecular Weight: 75,625 Da
• NCBI Official Full Name: xaa-Pro aminopeptidase 2
• NCBI Official Synonym Full Names: X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound
• NCBI Official Symbol: XPNPEP2
• NCBI Official Synonym Symbols: APP2; AEACEI
• NCBI Protein Information: xaa-Pro aminopeptidase 2; mAmP; membrane-bound APP; membrane-bound AmP; X-Pro aminopeptidase 2; aminoacylproline aminopeptidase; membrane-bound aminopeptidase P
• UniProt Protein Name: Xaa-Pro aminopeptidase 2
• UniProt Gene Name: XPNPEP2
• UniProt Synonym Gene Names: Membrane-bound APP; Membrane-bound AmP; mAmP
• UniProt Entry Name: XPP2_HUMAN

NCBI Description

Aminopeptidase P is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. Structurally, the enzyme is a member of the 'pita bread fold' family and occurs in mammalian tissues in both soluble and GPI-anchored membrane-bound forms. A membrane-bound and soluble form of this enzyme have been identified as products of two separate genes. [provided by RefSeq, Jul 2008]

Uniprot Description

XPNPEP2: A metalloprotease that may play a role in the inflammatory process and other reactions produced in response to injury or infection. May also play a role in the metabolism of the vasodilator bradykinin. Belongs to the peptidase M24B family.

Protein type: Membrane protein, GPI anchor; Protease; EC 3.4.11.9

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: membrane; plasma membrane

Molecular Function: metallopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: proteolysis

Disease: Angioedema Induced By Ace Inhibitors, Susceptibility To