Mouse anti-Human SerpinD1 Monoclonal Antibody | anti-SerpinD1 antibody

Anti-SerpinD1 Antibody, Mouse Monoclonal

Cat. Num. AAA8102146

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: SerpinD1; Monoclonal Antibody; Anti-SerpinD1 Antibody; Mouse Monoclonal; SerpinD1 Antibody; Mouse MAb; serpin peptidase inhibitor; clade D (heparin cofactor); member 1; Anti-D22S673 Antibody; Anti-HC2 Antibody; Anti-HCF2 Antibody; Anti-HCII Antibody; Anti-HLS2 Antibody; Anti-LS2 Antibody; Anti-THPH10 Antibody; anti-SerpinD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human SerpinD1.; No cross-reactivity in ELISA with Human SerpinC1, Human SerpinI1, Mouse SerpinD1, Mouse SerpinF1, Mouse SerpinF2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SerpinD1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: Recombinant Human SerpinD1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinD1 (rh SerpinD1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human serpinD1 in human liver with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)

Immunohistochemistry (IHC)

(Immunochemical staining of human serpinD1 in human hepatoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)

Related Product Information for anti-SerpinD1 antibody

SerpinD1, also known as heparin cofactor II, is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

References

Rau JC, et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study. Exp Mol Pathol. 87(3): 178-83.Aihara K, et al. (2009) Heparin cofactor II as a novel vascular protective factor against atherosclerosis. J Atheroscler Thromb. 16(5): 523-31.

NCBI and Uniprot Product Information

• NCBI GI #: 73858566
• NCBI GeneID: 3053
• NCBI Accession #: NP_000176.2
• NCBI GenBank Nucleotide #: NM_000185.3
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34
• Molecular Weight: 57,071 Da
• NCBI Official Full Name: heparin cofactor 2
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• NCBI Protein Information: heparin cofactor 2; serpin D1; leuserpin 2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2; HC-II; HLS2
• UniProt Entry Name: HEP2_HUMAN

NCBI Description

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; endopeptidase inhibitor activity

Biological Process: blood coagulation; chemotaxis

Disease: Heparin Cofactor Ii Deficiency