SDS-PAGE
SerpinD1 / HCII Recombinant Protein | HCII recombinant protein
Recombinant Mouse SerpinD1 / HCII Protein (His tag)
Gene Names
Serpind1; HCII; Hcf2; HC-II; AA985900; AI303446
Purity
> 98 % as determined by SDS-PAGE
Synonyms
SerpinD1 / HCII; Recombinant Mouse SerpinD1 / HCII Protein (His tag); Serpind1; AA985900; AI30344 6; HCII; Hcf2; MGC107662; HCII recombinant protein
Host
Human Cells
Purity/Purification
> 98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 25mM HEPES, 150mM NaCl, pH 7.4
Sequence Length
478
Application Notes
The recombinant mouse SerpinD1 consists of 466 amino acids and predicts a molecular mass of 53.5 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmSerpinD1 is approximately 65-70 kDa as a result of glycosylation.
Predicted N Terminal
Glu 24
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for HCII recombinant protein
Background: SerpinD1, also known as heparin cofactor II (HCII), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans (GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Description: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,497 Da
NCBI Official Full Name
heparin cofactor 2
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade D, member 1
NCBI Official Symbol
Serpind1
NCBI Official Synonym Symbols
HCII; Hcf2; HC-II; AA985900; AI303446
NCBI Protein Information
heparin cofactor 2
UniProt Protein Name
Heparin cofactor 2
UniProt Gene Name
Serpind1
UniProt Synonym Gene Names
Hcf2; Hcii; HC-II
Uniprot Description
SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.
Chromosomal Location of Human Ortholog: 16 A3|16 10.74 cM
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on HCII
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Product Notes
The HCII serpind1 (Catalog #AAA2547151) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse SerpinD1 consists of 466 amino acids and predicts a molecular mass of 53.5 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmSerpinD1 is approximately 65-70 kDa as a result of glycosylation. Researchers should empirically determine the suitability of the HCII serpind1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SerpinD1 / HCII, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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