SERPIND1 recombinant protein

Recombinant Human SerpinD1 Protein (His tag)

Cat. Num. AAA2546355

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SerpinD1; Recombinant Human SerpinD1 Protein (His tag); HC2; LS2; HCF2; HCII; HLS2; D22S673; SERPIND1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 499

• Application Notes: The mature recombinant human SerpinD1 consists of 491 amino acids and predicts a molecular mass of 56.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhSerpinD1 is approximately 65-70 kDa as a result of glycosylation.
• Predicted N Terminal: Gly 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SERPIND1 recombinant protein

Background: SerpinD1, also known as heparin cofactor II (HCII), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans (GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Description: A DNA sequence encoding the human SerpinD1 precursor (NP_000176.2) (Met 1-Ser 499) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 73858566
• NCBI GeneID: 3053
• NCBI Accession #: NP_000176.2
• NCBI GenBank Nucleotide #: NM_000185.3
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34
• Molecular Weight: 57,071 Da
• NCBI Official Full Name: heparin cofactor 2
• NCBI Official Synonym Full Names: serpin family D member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• NCBI Protein Information: heparin cofactor 2
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2; HC-II; HLS2

NCBI Description

This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]

Uniprot Description

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular region; extracellular space

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: blood coagulation

Disease: Heparin Cofactor Ii Deficiency