CD24 recombinant protein

Recombinant Human CD24 Protein (Fc tag)

Cat. Num. AAA2545954

• Gene Names: CD24; CD24A
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: CD24; Recombinant Human CD24 Protein (Fc tag); CD24A; FLJ22950; FLJ43543; MGC75043; CD24 recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 80

• Application Notes: The recombinant human CD24/Fc is a disulfide-linked homodimer. The reduced monomer consists of 274 amino acids and has a predicted molecular mass of 30 kDa. As a result of glycosylation, rh CD24/Fc monomer migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD24 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 24, also known as signal transducer CD24 or heat stable antigen CD24 (HSA), is a mucin-type glycosylphosphatidylinositol-linked glycoprotein expressed on the surface of B-cells, differentiating neuroblasts and many tumors. It is involved in molecular adhesion and metastatic tumor spread and serve as a normal receptor for P-selectin. The CD24 / P-selectin pathway could be important in dissimenating of tumor cells by facilitating the interaction with platelet and endothelial cells. It has also been considered as a tumor marker. High rate of CD24 expressions have been found in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer.

Description: A DNA sequence encoding the human CD24 (NP_037362.1) (Met 1-Gly 59) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7019343
• NCBI GeneID: 100133941
• NCBI Accession #: NP_037362.1
• NCBI GenBank Nucleotide #: NM_013230.3
• UniProt Accession #: P25063; Q16257; Q53XS0
• Molecular Weight: 8,097 Da
• NCBI Official Full Name: signal transducer CD24 isoform a preproprotein
• NCBI Official Synonym Full Names: CD24 molecule
• NCBI Official Symbol: CD24
• NCBI Official Synonym Symbols: CD24A
• NCBI Protein Information: signal transducer CD24
• UniProt Protein Name: Signal transducer CD24
• Protein Family: Signal transducer
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Cell development/differentiation; Cell surface; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: anchored to external side of plasma membrane; cell surface; lipid raft; membrane

Molecular Function: protein binding; protein kinase binding; protein tyrosine kinase activator activity

Biological Process: B cell receptor transport into lipid raft; cell activation; cell migration; chemokine receptor transport out of lipid raft; cholesterol homeostasis; elevation of cytosolic calcium ion concentration; immune response-regulating cell surface receptor signaling pathway; negative regulation of transforming growth factor-beta3 production; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; regulation of cytokine and chemokine mediated signaling pathway; regulation of MAPKKK cascade; regulation of phosphorylation; respiratory burst; response to estrogen stimulus; response to hypoxia; response to molecule of bacterial origin; T cell costimulation

Disease: Multiple Sclerosis, Susceptibility To