NBL1 active protein

NBL1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120046

• Gene Names: NBL1; NB; DAN; NO3; DAND1; D1S1733E
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NBL1; NBL1 Protein; Human; Recombinant (hFc Tag); Human NBL1/DAND1/DAN Protein (Fc Tag); D1S1733E Protein; DAN Protein; DAND1 Protein; NB Protein; NO3 Protein; neuroblastoma 1; DAN family BMP antagonist; NBL1 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp180

• Species: Human
• Activity: Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1.2ug/ml in the presence of 50ng/mL of recombinant human BMP4.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 16
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human DAN precursor (NP_005371.1)(Met 1-Asp 180) was expressed with C-terminal fused Fc region of human IgG1.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1.2 ug/ml in the presence of 50 ng/mL of recombinant human BMP4.)

Related Product Information for NBL1 active protein

Background: The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.

References

Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.

NCBI and Uniprot Product Information

• NCBI GI #: 4885509
• NCBI GeneID: 4681
• NCBI Accession #: NP_005371.1
• NCBI GenBank Nucleotide #: NM_005380.4
• Molecular Weight: 23,169 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1 isoform 2
• NCBI Official Synonym Full Names: neuroblastoma 1, DAN family BMP antagonist
• NCBI Official Symbol: NBL1
• NCBI Official Synonym Symbols: NB; DAN; NO3; DAND1; D1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• Protein Family: N-terminal-borealin-like protein

NCBI Description

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]