EGFR active protein

EGFR Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123116

• Gene Names: Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EGFR; EGFR Protein; Mouse; Recombinant (His Tag); Mouse EGFR/HER1/ErbB1 Protein (His Tag); 9030024J15Rik Protein; AI552599 Protein; Erbb Protein; Errb1 Protein; Errp Protein; wa-2 Protein; wa2 Protein; Wa5 Protein; epidermal growth factor receptor; EGFR active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser647

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279)(Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized mouse EGFR-his at 10ug/mL (100uL/well) can bind human EGF-Fc. The EC50 of human EGF-Fc is 60-90ng/mL.; 3. Immobilized mouse EGFR-his at 10ug/mL (100uL/well) can bind mouse EGF-Fc. The EC50 of mouse EGF-Fc is 70-100ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. 1. Immobilized mouse EGFR-his at 10 ug/mL (100 ul/well) can bind human EGF-Fc, The EC50 of human EGF-Fc is 60-90 ng/mL. 2. Immobilized mouse EGFR-his at 10 ug/mL (100 ul/well) can bind mouse EGF-Fc, The EC50 of mouse EGF-Fc is 70-100 ng/mL.)

Related Product Information for EGFR active protein

Background: As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Product Categories/Family for EGFR active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs); EGFR Signaling Pathway

References

Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

NCBI and Uniprot Product Information

• NCBI GI #: 46560582
• NCBI GeneID: 13649
• NCBI Accession #: NP_997538.1
• NCBI GenBank Nucleotide #: NM_207655.2
• UniProt Accession #: Q01279
• Molecular Weight: 134,853 Da
• NCBI Official Full Name: epidermal growth factor receptor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: Egfr
• NCBI Official Synonym Symbols: Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• Protein Family: Epidermal growth factor receptor
• UniProt Gene Name: Egfr
• UniProt Entry Name: EGFR_MOUSE

Uniprot Description

EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Tumor suppressor; Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; EGFR family

Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; cytoplasm; early endosome membrane; endocytic vesicle; endoplasmic reticulum; endosome; endosome membrane; focal adhesion; Golgi apparatus; integral to membrane; intracellular; lipid raft; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex; synapse

Molecular Function: actin filament binding; ATP binding; calmodulin binding; chromatin binding; enzyme binding; epidermal growth factor binding; epidermal growth factor receptor activity; glycoprotein binding; identical protein binding; integrin binding; kinase activity; nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein kinase binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; signal transducer activity; transferase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: activation of MAPKK activity; astrocyte activation; cell morphogenesis; cell proliferation; cell surface receptor linked signal transduction; cell-cell adhesion; cerebral cortex cell migration; digestive tract morphogenesis; embryonic placenta development; epidermal growth factor receptor signaling pathway; epidermis development; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; hair follicle development; learning and/or memory; multicellular organismal development; negative regulation of apoptosis; negative regulation of mitotic cell cycle; negative regulation of protein catabolic process; neurite morphogenesis; ovulation cycle; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of bone resorption; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA repair; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of superoxide release; positive regulation of synaptic transmission, glutamatergic; positive regulation of transcription from RNA polymerase II promoter; positive regulation of vasoconstriction; positive regulation of vasodilation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of nitric-oxide synthase activity; regulation of peptidyl-tyrosine phosphorylation; response to calcium ion; salivary gland morphogenesis; signal transduction; translation; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing