Kallikrein 11 Active Protein | KLK11 active protein

Recombinant Human Kallikrein 11 Protein

Cat. Num. AAA2556701

• Gene Names: KLK11; TLSP; PRSS20
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Kallikrein 11; Recombinant Human Kallikrein 11 Protein; Kallikrein-11; hK11; Hippostasin; Serine Protease 20; Trypsin-Like Protease; KLK11; PRSS20; TLSP; KLK11 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM TrisHCl, 150mM NaCl, 2mM CaCl2, pH 8.0.
• Sequence Length: 250

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Glu19-Asn250
• Fusion Tag: C-6His
• Activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468). The specific activity is >200 pmoles/min/mug.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

SDS-Page

Related Product Information for KLK11 active protein

Protein Construction: Recombinant Human Kallikrein 11 is produced by our Mammalian expression system and the target gene encoding Glu19-Asn250 is expressed with a 6His tag at the C-terminus.

Background: Human Kallikrein 11 (KLK11) is a member of tissue kallikrein family which are extracellular serine proteases consisting of 15 members. Two isoforms of KLK11 are differentially expressed. Isoform 1 is predominantly expressed in brain and isoform 2 is preferentially expressed in prostate. Isoform 1 consists of a signal peptide,a short pro peptide and the mature chain.Isoform 2 contains an extra 32 amino acid N terminal to full-length isoform 1.KLK11 is a novel marker for ovarian and prostate cancer carcinomas. KLK11 can be activated by thermolysin and is active against a thioester substrate.

NCBI and Uniprot Product Information

• NCBI GI #: 5803199
• NCBI GeneID: 11012
• NCBI Accession #: NP_006844.1
• NCBI GenBank Nucleotide #: NM_006853.2
• UniProt Accession #: Q9UBX7; O75837; Q0WXX5; Q8IXD7; Q9NS65
• Molecular Weight: Molecular Mass: 26.3kDa; Actual Protein Molecular Mass: 38kDa
• NCBI Official Full Name: kallikrein-11 isoform 1
• NCBI Official Synonym Full Names: kallikrein related peptidase 11
• NCBI Official Symbol: KLK11
• NCBI Official Synonym Symbols: TLSP; PRSS20
• NCBI Protein Information: kallikrein-11
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing and the use of alternate promoters results in multiple transcript variants encoding distinct isoforms which are differentially expressed. [provided by RefSeq, Dec 2016]

Uniprot Description

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.