Goat STUB1 Polyclonal Antibody | anti-STUB1 antibody

STUB1 Polyclonal Antibody

Cat. Num. AAA448144

• Gene Names: STUB1; CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
• Reactivity: Human, Rat, Mouse, Canine, Monkey proteins
• Applications: Western Blot
• Purity: Epitope Affinity Purified
• Synonyms: STUB1; Polyclonal Antibody; STUB1 Polyclonal Antibody; STIP1 homology and U-box containing protein 1; E3 ubiquitin protein ligase; Anti-STUB1; CHIP; HSPABP2; NY-CO-7; SCAR16; SDCCAG7; UBOX1 antibody; anti-STUB1 antibody

• Host: Goat
• Reactivity: Human, Rat, Mouse, Canine, Monkey proteins
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of STUB1 by Western blot.
• Purity/Purification: Epitope Affinity Purified
• Form/Format: Supplied as a 100 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Sequence Length: 231

• Applicable Applications for anti-STUB1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:5,000
• Immunogen: Recombinant human STUB1 protein produced in E Coli.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.; The antibody solution should be gently mixed before use.; Avoid freeze/thaw cycles.

Reactivity Chart

Wester Blot (WB)

Related Product Information for anti-STUB1 antibody

Goat polyclonal antibody to STUB1. STUB1 protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. It binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets.

NCBI and Uniprot Product Information

• NCBI GI #: 645912990
• NCBI GeneID: 10273
• NCBI Accession #: NP_001280126.1
• NCBI GenBank Nucleotide #: NM_001293197.1
• UniProt Accession #: Q9UNE7; O60526; Q969U2; Q9HBT1; A2IDB9
• Molecular Weight: 27,067 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CHIP isoform b
• NCBI Official Synonym Full Names: STIP1 homology and U-box containing protein 1
• NCBI Official Symbol: STUB1
• NCBI Official Synonym Symbols: CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
• NCBI Protein Information: E3 ubiquitin-protein ligase CHIP
• UniProt Protein Name: E3 ubiquitin-protein ligase CHIP
• Protein Family: STIP1 homology and U box-containing protein
• UniProt Gene Name: STUB1

NCBI Description

This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014]

Uniprot Description

CHIP: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Homodimer. Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DYX1C1 and POLB. Interacts (via TPR repeats) with HSP90AA1. Interacts (when monoubiquitinated) with ATXN3. Interacts with UBE2W. Interacts (via the U-box domain) with the UBE2V2- UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with DNAJB6. Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; nuclear inclusion body; nucleoplasm; ubiquitin conjugating enzyme complex; ubiquitin ligase complex

Molecular Function: enzyme binding; G-protein-coupled receptor binding; Hsp70 protein binding; Hsp90 protein binding; kinase binding; misfolded protein binding; protein binding; protein binding, bridging; protein homodimerization activity; SMAD binding; TPR domain binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: misfolded or incompletely synthesized protein catabolic process; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein maturation; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of glucocorticoid metabolic process; regulation of protein stability; ubiquitin-dependent protein catabolic process; ubiquitin-dependent SMAD protein catabolic process

Disease: Spinocerebellar Ataxia, Autosomal Recessive 16