CD70 active protein

CD70 Protein, Mouse, Recombinant (mFc Tag)

Cat. Num. AAA8123154

• Gene Names: Cd70; Cd27l; CD27LG; Tnfsf7
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD70; CD70 Protein; Mouse; Recombinant (mFc Tag); Mouse CD70/CD27L/TNFSF7 Protein (Fc Tag); Cd27l Protein; CD27LG Protein; Tnfsf7 Protein; CD70 molecule; CD70 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln47-Pro159

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Cd70 (NP_035747.1)(Gln47-Pro195) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1.Measured by its binding ability in a functional ELISA.2.Immobilized mouse CD27-His at 10ug/mL (100uL/well) can bind mouse CD70. The EC50 of mouse CD70 is 10-50ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse CD27-His at 10 ug/mL (100 uL/well) can bind mouse CD70, the EC50of mouse CD70 is 10-50 ng/mL.)

Related Product Information for CD70 active protein

Background: CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.

Product Categories/Family for CD70 active protein

Cancer Drug Targets

References

Adam PJ, et al. (2006) CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding. Br J Cancer. 95(3): 298-306.Keller AM, et al. (2007) Costimulatory ligand CD70 is delivered to the immunological synapse by shared intracellular trafficking with MHC class II molecules. Proc Natl Acad Sci U S A. 104(14): 5989-94.Grewal IS. (2008) CD70 as a therapeutic target in human malignancies. Expert Opin Ther Targets. 12(3): 341-51.Boursalian TE, et al. (2009) Targeting CD70 for human therapeutic use. Adv Exp Med Biol. 647: 108-19.

NCBI and Uniprot Product Information

• NCBI GI #: 6755839
• NCBI GeneID: 21948
• NCBI Accession #: NP_035747.1
• NCBI GenBank Nucleotide #: NM_011617.2
• UniProt Accession #: O55237
• Molecular Weight: 21,919 Da
• NCBI Official Full Name: CD70 antigen
• NCBI Official Synonym Full Names: CD70 antigen
• NCBI Official Symbol: Cd70
• NCBI Official Synonym Symbols: Cd27l; CD27LG; Tnfsf7
• NCBI Protein Information: CD70 antigen; CD27 ligand; CD27-L; Ki-24 antigen; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand superfamily member 7
• UniProt Protein Name: CD70 antigen
• Protein Family: CD70 antigen
• UniProt Gene Name: Cd70
• UniProt Synonym Gene Names: Cd27l; Cd27lg; Tnfsf7; CD27-L
• UniProt Entry Name: CD70_MOUSE

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane

Molecular Function: protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction