Typical Testing Data/Standard Curve (for reference only)
(Mouse CD70/TNFSF7/cd27L PicoKine ELISA Kit standard curve )
Mouse CD70/TNFSF7/cd27L ELISA Kit | CD70 elisa kit
Mouse CD70/TNFSF7/cd27L PicoKine ELISA Kit
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7
Reactivity
Mouse
Synonyms
CD70/TNFSF7/cd27L; Mouse CD70/TNFSF7/cd27L PicoKine ELISA Kit; CD70 antigen; CD70 molecule; CD 27L; CD 70; CD27 L; CD27 LG; CD27 ligand; CD27-L; CD27L; CD27LG; CD70; CD70_HUMAN; Ki 24 antigen; Ki24 antigen; Surface antigen CD70; TNFSF 7; TNFSF7; Tumor necrosis factor (ligand) superfamily; member 7; Tumor necrosis factor ligand superfamily member; CD70 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD70
Sequence Length
195
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD70/TNFSF7/cd27L PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD70/TNFSF7/cd27L PicoKine ELISA Kit standard curve )
Related Product Information for CD70 elisa kit
Principle of the assay: mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been precoated onto 96-well plates. Standards(NSO, S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD70 amount of sample captured in plate.
Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,919 Da
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 antigen
NCBI Official Symbol
Cd70
NCBI Official Synonym Symbols
Cd27l; CD27LG; Tnfsf7
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name
CD70 antigen
Protein Family
UniProt Gene Name
Cd70
UniProt Synonym Gene Names
Cd27l; Cd27lg; Tnfsf7; CD27-L
UniProt Entry Name
CD70_MOUSE
Uniprot Description
CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction
Research Articles on CD70
Similar Products
Product Notes
The Mouse CD70 cd70 (Catalog #AAA177208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177208 ELISA Kit recognizes Mouse CD70. It is sometimes possible for the material contained within the vial of "CD70/TNFSF7/cd27L, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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