Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Methyltransferase like 5 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Rabbit Methyltransferase like 5 Polyclonal Antibody | anti-METTL5 antibody
Methyltransferase like 5 Antibody
Gene Names
METTL5; MRT72; HSPC133
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%), Xenopus(88%)
Applications
Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Methyltransferase like 5; Polyclonal Antibody; Methyltransferase like 5 Antibody; 2810410A08Rik; DC3; FLJ10459; HSPC133; mCG_12967; Methyltransferase like protein 5; Methyltransferase-like protein 5; METL5_HUMAN; METTL 5; METTL5; MGC187943; OTTHUMP00000205107; OTTHUMP00000205108; OTTHUMP00000205109; OTTMUSP00000040951; RGD1566062; anti-METTL5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Methyltransferase like 5 Antibody detects endogenous levels of total Methyltransferase like 5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
209
Applicable Applications for anti-METTL5 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Methyltransferase like 5, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Methyltransferase like 5 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Methyltransferase like 5 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Immunohistochemistry (IHC)-Paraffin
(MBS9611991 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9611991 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9611991 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9611991 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9611991 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9611991 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-METTL5 antibody
Function: Probable methyltransferase.
Similarity: Belongs to the methyltransferase superfamily. PrmA family.
Similarity: Belongs to the methyltransferase superfamily. PrmA family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)23kD, 40kD.
Predicted Molecular Weight: (Calculated)24kDa.
Predicted Molecular Weight: (Calculated)24kDa.
NCBI Official Full Name
rRNA N6-adenosine-methyltransferase METTL5
NCBI Official Synonym Full Names
methyltransferase like 5
NCBI Official Symbol
METTL5
NCBI Official Synonym Symbols
MRT72; HSPC133
NCBI Protein Information
rRNA N6-adenosine-methyltransferase METTL5
UniProt Protein Name
Methyltransferase-like protein 5
Protein Family
UniProt Gene Name
METTL5
UniProt Synonym Gene Names
DC3; HSPC133
UniProt Entry Name
METL5_HUMAN
Research Articles on METTL5
Similar Products
Product Notes
The METTL5 mettl5 (Catalog #AAA9611991) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Methyltransferase like 5 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%), Xenopus(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Methyltransferase like 5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the METTL5 mettl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methyltransferase like 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
