Typical Testing Data/Standard Curve (for reference only)
Human Ribonuclease L (RNASEL) ELISA Kit | RNASEL elisa kit
Human Ribonuclease L (RNASEL) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to RNASEL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RNASEL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNASEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]
Uniprot Description
RNASEL: a multifunctional enzyme that possesses protein kinase and endoribonuclease activity, Mediator of interferon action, which play a role in mediating resistance to virus infection and apoptosis. Might play a central role in the regulation of mRNA turnover.
Protein type: Ribonuclease; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 3.1.26.-; Other group; Other-Unique family
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: nuclear matrix; mitochondrion; cytosol
Molecular Function: endoribonuclease activity; rRNA binding; protein binding; RNA binding; metal ion binding; ATP binding; protein kinase activity
Biological Process: negative regulation of viral genome replication; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; mRNA processing; protein amino acid phosphorylation; defense response to virus; rRNA processing
Disease: Prostate Cancer, Hereditary, 1
Research Articles on RNASEL
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Product Notes
The Human RNASEL rnasel (Catalog #AAA2019751) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019751 ELISA Kit recognizes Human RNASEL. It is sometimes possible for the material contained within the vial of "Ribonuclease L (RNASEL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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