Mouse Cathepsin Z (CTSZ) ELISA Kit | CTSZ elisa kit

Mouse Cathepsin Z (CTSZ) ELISA Kit

Cat. Num. AAA455784

• Gene Names: Ctsz; CTSX; AI787083; AU019819; D2Wsu143e
• Reactivity: Mouse
• Synonyms: Cathepsin Z (CTSZ); Mouse Cathepsin Z (CTSZ) ELISA Kit; CTS-Z; CTSX; Cathepsin P; Cathepsin X; CTSZ elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTSZ. No significant cross-reactivity or interference between CTSZ and analogues was observed.
• Sequence Length: 306

• Samples: Serum, plasma or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 7.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTSZ were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTSZ were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSZ elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CTSZ in mouse serum, plasma or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CTSZ. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CTSZ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTSZ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CTSZ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11968166
• NCBI GeneID: 64138
• NCBI Accession #: NP_071720.1
• NCBI GenBank Nucleotide #: NM_022325.5
• UniProt Accession #: Q9WUU7
• Molecular Weight: 33,996 Da
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: Ctsz
• NCBI Official Synonym Symbols: CTSX; AI787083; AU019819; D2Wsu143e
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsz

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate a mature enzyme with carboxypeptidase activity. An enzymatically inactive form of the protein, that is associated with the propeptide, may be involved in cancer cell invasion and proliferation. Homozygous knockout mice for this gene exhibit impaired cancer cell invasion in a breast cancer model. [provided by RefSeq, Aug 2015]

Uniprot Description

Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.