SDS-PAGE
(Fig A. SDS-PAGE (4-20%) of Recombinant GZMB: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue.The protein migrates as 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.)
Granzyme B Active Protein | GZMB active protein
Human CellExp Granzyme B, human recombinant
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Granzyme B; Human CellExp Granzyme B; human recombinant; GZMB; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; Fragmentin-2; Granzyme-2; GRB; GZMB active protein
Host
HEK 293 cells (Ile 21-Tyr 247)
Specificity
Specific Reactivity > = 5 mU/mg.
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Lyophilized powder from 0.22 um filtered solution in PBS. containing 8% trehalose
Applicable Applications for GZMB active protein
SDS-PAGE; HPLC
Application Notes
Active GZMB is useful in studying enzyme regulation, determining target substrates, screening GZMB inhibitors, or as a positive control in GZMB activity assays
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4. Do not vortex. Store the solution in aliquots at -20°C for long term storage.
Unit Definition
One unit of Granzyme B is defined as the amount of enzyme that hydrolyzes 1 umol of Ac-IEPD-AFC per min at 37 °C under the assay conditions (100 uM substrate Ac-IETD-AFC in a buffer containing 50 mM HEPES pH 7.5, 10 mM CaCl2).
Biological Activity
Biological activity was performed using Granzyme B Activity Fluorometric Assay Kit.
Preparation and Storage
Store at -20 °C. After reconstitution, aliquot and store at -20 °C and use within 3 months. Avoid repeated freeze-thaw cycles.
SDS-PAGE
(Fig A. SDS-PAGE (4-20%) of Recombinant GZMB: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue.The protein migrates as 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.)
SDS-PAGE
(Fig A. SDS-PAGE (4-20%) of Recombinant GZMB: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue.The protein migrates as 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.)
Specific activity
(Fig B. Specific activity: Assay was performed using Granzyme B Activity Fluorometric Assay Kit (Cat #: MBS840001).The activity of GZMB was determined using 100 uM substrate Ac-IETD-AFC in a buffer containing 50 mM HEPES pH 7.5, 10 mM CaCl2 at 37°C.)
Specific activity
(Fig B. Specific activity: Assay was performed using Granzyme B Activity Fluorometric Assay Kit (Cat #: MBS840001).The activity of GZMB was determined using 100 uM substrate Ac-IETD-AFC in a buffer containing 50 mM HEPES pH 7.5, 10 mM CaCl2 at 37°C.)
Related Product Information for GZMB active protein
Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. Elevated abundance of Granzyme B is also implicated in autoimmune diseases, skin disorders and type 1 diabetes.
Product Categories/Family for GZMB active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GZMB (Catalog #AAA844591) is an Active Protein produced from HEK 293 cells (Ile 21-Tyr 247) and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Granzyme B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC. Active GZMB is useful in studying enzyme regulation, determining target substrates, screening GZMB inhibitors, or as a positive control in GZMB activity assays. Researchers should empirically determine the suitability of the GZMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granzyme B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
