Mouse anti-Human Granzyme B Monoclonal Antibody | anti-GZMB antibody

Anti-Granzyme B Antibody, Mouse Monoclonal

Cat. Num. AAA8102376

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A
• Synonyms: Granzyme B; Monoclonal Antibody; Anti-Granzyme B Antibody; Mouse Monoclonal; Granzyme B/GZMB Antibody; Mouse MAb; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1); Anti-CCPI Antibody; Anti-CGL-1 Antibody; Anti-CGL1 Antibody; Anti-CSP-B Antibody; Anti-CSPB Antibody; Anti-CTLA1 Antibody; Anti-CTSGL1 Antibody; Anti-HLP Antibody; Anti-SECT Antibody; anti-GZMB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 2F5F2D10
• Specificity: Human Granzyme B/GZMB
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GZMB antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:1000-1:2000; IHC-P: 1:50-1:200
• Immunogen: Recombinant Human Granzyme B protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme B (rhGranzyme B). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human Granzyme B in human tonsil with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human Granzyme B in human spleen with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human Granzyme B in human lymph node with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-GZMB antibody

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.

Product Categories/Family for anti-GZMB antibody

Serine Proteases and Regulators

References

Schmid J., et al., (1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.Trapani J.A., et al., (1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.

NCBI and Uniprot Product Information

• NCBI GI #: 4758494
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.1
• NCBI GenBank Nucleotide #: NM_004131.3
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: granzyme B
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• Protein Family: Granzyme

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]