Granzyme B (Insect) Active Protein | Granzyme B active protein

Granzyme B, human recombinant (Insect)

Cat. Num. AAA845325

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Granzyme B (Insect); Granzyme B; human recombinant (Insect); GranzymeB; Granzyme-2; T-cell serine protease 1-3E; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; SECT; Cathepsin G-like 1; CTSGL1; CTLA-1; Fragmentin-2; Human lymphocyte protein; HLP; C11; Granzyme B active protein

• Host: Insect cells
• Purity/Purification: >=90%
• Form/Format: Lyophilized from 20 mM HEPES buffer at pH 7.5.; Appearance: Lyophilized protein
• Sequence Length: 247

• Applicable Applications for Granzyme B active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Use 50 ul sterile D.I. water to dissolve 5 ug protein to a final conc of 0.1 ug protein/ul.
• Biological Activity: Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.
• Results: >=40 units/mug
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -70 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for Granzyme B active protein

Background: Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

Product Categories/Family for Granzyme B active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 181186
• NCBI GeneID: 3002
• Molecular Weight: 32 kDa
• NCBI Official Full Name: granzyme B
• NCBI Official Synonym Full Names: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Protease; Apoptosis; EC 3.4.21.79

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; immune response; protein processing; proteolysis