Hemagglutinin-esterase (HE) Recombinant Protein | HE recombinant protein

Recombinant Bovine coronavirus Hemagglutinin-esterase (HE)

Cat. Num. AAA1120206

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Hemagglutinin-esterase (HE); Recombinant Bovine coronavirus Hemagglutinin-esterase (HE); E3 glycoprotein; HE recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 19-424. Full Length of Mature Protein
• Sequence: FDNPPTNVVSHLNGDWFLFGDSRSDCNHVVNTNPRNYSYMDLNPALCDSGKISSKAGNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCTTSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQSTALCKSGSLVLNNPAYIAREANFGDYYYKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDDSQYYFNKDTGVIYGLNSTETITTGFDFNCHYLVLPSGNYLAISNELLLTVPTKAICLNKRKDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYDSPCFSQQGVFRYDNVSSVWPLYSYGRCPTAADINTPDVPICVYDPLPLILLGILLGVAVIIIVVLLLYFMVDNGTRLHDA

• Species: BCoV
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Production Note: Special Offer: The Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Related Product Information for HE recombinant protein

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Ses to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

References

Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein.Kienzle T.E., Abraham S., Hogue B.G., Brian D.A.J. Virol. 64:1834-1838(1990) Structure and expression of the bovine coronavirus hemagglutinin protein.Kienzle T.E., Abraham S., Hogue B.G., Brian D.A.Adv. Exp. Med. Biol. 276:95-102(1990) Synthesis and processing of the bovine enteric coronavirus haemagglutinin protein.Hogue B.G., Kienzle T.E., Brian D.A.J. Gen. Virol. 70:345-352(1989) Nidovirus sialate-O-acetylesterases evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes.Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.J. Biol. Chem. 280:6933-6941(2005) Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution.Zeng Q., Langereis M.A., van Vliet A.L., Huizinga E.G., de Groot R.J.Proc. Natl. Acad. Sci. U.S.A. 105:9065-9069(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 122851
• UniProt Accession #: P15776
• Molecular Weight: 47.7 kDa
• NCBI Official Full Name: Hemagglutinin-esterase
• UniProt Protein Name: Hemagglutinin-esterase
• Protein Family: Hemagglutinin-esterase
• UniProt Gene Name: HE
• UniProt Synonym Gene Names: HE protein
• UniProt Entry Name: HEMA_CVBM

Uniprot Description

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.