Human Regulator Of G Protein Signaling 1 (RGS1) ELISA Kit | RGS1 elisa kit

Human Regulator Of G Protein Signaling 1 (RGS1) ELISA Kit

Cat. Num. AAA2024994

• Gene Names: RGS1; 1R20; BL34; IER1; IR20; HEL-S-87
• Reactivity: Human
• Synonyms: Regulator Of G Protein Signaling 1 (RGS1); Human Regulator Of G Protein Signaling 1 (RGS1) ELISA Kit; 1R20; BL34; IER1; IR20; B-cell activation protein BL34; Early response protein 1R20; RGS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 1 (RGS1).; No significant cross-reactivity or interference between Regulator Of G Protein Signaling 1 (RGS1) and analogues was observed.
• Sequence Length: 209

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 1 (RGS1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 1 (RGS1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 1 (RGS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 1 (RGS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 1 (RGS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 1 (RGS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RGS1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 115387108
• NCBI GeneID: 5996
• NCBI Accession #: NP_002913.3
• NCBI GenBank Nucleotide #: NM_002922.3
• Molecular Weight: 19,134 Da
• NCBI Official Full Name: regulator of G-protein signaling 1
• NCBI Official Synonym Full Names: regulator of G-protein signaling 1
• NCBI Official Symbol: RGS1
• NCBI Official Synonym Symbols: 1R20; BL34; IER1; IR20; HEL-S-87
• NCBI Protein Information: regulator of G-protein signaling 1
• UniProt Protein Name: Regulator of G-protein signaling 1
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS1
• UniProt Synonym Gene Names: 1R20; BL34; IER1; RGS1
• UniProt Entry Name: RGS1_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS1: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. This protein may be involved in the regulation of B-cell activation and proliferation. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calmodulin binding; GTPase activator activity

Biological Process: immune response; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; positive regulation of GTPase activity