Typical Standard Curve/Testing Data
Human Hyaluronate binding protein ELISA Kit | HABP elisa kit
Human Hyaluronate binding protein ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: HABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HABP antibody and an HABP-HRP conjugate. The assay sample and buffer are incubated together with HABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HABP concentration since HABP from samples and HABP-HRP conjugate compete for the anti-HABP antibody binding site. Since the number of sites is limited, as more sites are occupied by HABP from the sample, fewer sites are left to bind HABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HABP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]
Uniprot Description
TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
Chromosomal Location of Human Ortholog: 2q23.3
Cellular Component: extracellular space
Molecular Function: hyaluronic acid binding
Biological Process: ovulation; cell-cell signaling; inflammatory response; cell adhesion; signal transduction; positive regulation of cell migration
Research Articles on HABP
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Product Notes
The Human HABP tnfaip6 (Catalog #AAA733122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733122 ELISA Kit recognizes Human HABP. It is sometimes possible for the material contained within the vial of "Hyaluronate binding protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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