Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Tumor Necrosis Factor-Inducible Gene 6 Protein ELISA Kit | TSG-6 elisa kit
Guinea Pig Tumor Necrosis Factor-Inducible Gene 6 Protein ELISA Kit
Gene Names
TNFAIP6; TSG6; TSG-6
Reactivity
Guinea Pig
Synonyms
Tumor Necrosis Factor-Inducible Gene 6 Protein; Guinea Pig Tumor Necrosis Factor-Inducible Gene 6 Protein ELISA Kit; TSG-6 elisa kit
Reactivity
Guinea Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of TNFIP6. No significant cross-reactivity or interference between TNFIP6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TNFIP6 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
277
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TSG-6 elisa kit
Intended Uses: This TNFIP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TNFIP6. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: TNFIP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TNFIP6 antibody and an TNFIP6-HRP conjugate. The assay sample and buffer are incubated together with TNFIP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFIP6 concentration since TNFIP6 from samples and TNFIP6-HRP conjugate compete for the anti-TNFIP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFIP6 from the sample, fewer sites are left to bind TNFIP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFIP6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: TNFIP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TNFIP6 antibody and an TNFIP6-HRP conjugate. The assay sample and buffer are incubated together with TNFIP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFIP6 concentration since TNFIP6 from samples and TNFIP6-HRP conjugate compete for the anti-TNFIP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFIP6 from the sample, fewer sites are left to bind TNFIP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFIP6 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TSG-6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,203 Da
NCBI Official Full Name
tumor necrosis factor-inducible gene 6 protein
NCBI Official Synonym Full Names
TNF alpha induced protein 6
NCBI Official Symbol
TNFAIP6
NCBI Official Synonym Symbols
TSG6; TSG-6
NCBI Protein Information
tumor necrosis factor-inducible gene 6 protein
UniProt Protein Name
Tumor necrosis factor-inducible gene 6 protein
UniProt Gene Name
TNFAIP6
UniProt Synonym Gene Names
TSG6; TSG-6; TNF alpha-induced protein 6
NCBI Description
The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]
Uniprot Description
Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
Research Articles on TSG-6
Similar Products
Product Notes
The Guinea Pig TSG-6 tnfaip6 (Catalog #AAA7258204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7258204 ELISA Kit recognizes Guinea Pig TSG-6. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor-Inducible Gene 6 Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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