Human tumor necrosis factor, alpha-induced protein 6 ELISA Kit | TNFAIP6 elisa kit

Human Tumor necrosis factor-inducible gene 6 protein, TNFAIP6 ELISA Kit

Cat. Num. AAA926793

• Gene Names: TNFAIP6; TSG6; TSG-6
• Reactivity: Human
• Synonyms: tumor necrosis factor; alpha-induced protein 6; Human Tumor necrosis factor-inducible gene 6 protein; TNFAIP6 ELISA Kit; Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA kit; TSG-6; TSG6; hyaluronate-binding protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein; TNFAIP6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TNFAIP6. No significant cross-reactivity or interference between human TNFAIP6 and analogues was observed.
• Sequence Length: 277

• Samples: Serum, plasma, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFAIP6 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 26051243
• NCBI GeneID: 7130
• NCBI Accession #: NP_009046.2
• NCBI GenBank Nucleotide #: NM_007115.3
• UniProt Accession #: P98066; Q53TI7; Q8WWI9
• Molecular Weight: 31,203 Da
• NCBI Official Full Name: tumor necrosis factor-inducible gene 6 protein
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 6
• NCBI Official Symbol: TNFAIP6
• NCBI Official Synonym Symbols: TSG6; TSG-6
• NCBI Protein Information: tumor necrosis factor-inducible gene 6 protein; TNF alpha-induced protein 6; hyaluronate-binding protein; TNF-stimulated gene 6 protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein
• UniProt Protein Name: Tumor necrosis factor-inducible gene 6 protein
• Protein Family: Tumor necrosis factor-inducible gene 6 protein
• UniProt Gene Name: TNFAIP6
• UniProt Synonym Gene Names: TSG6; TSG-6; TNF alpha-induced protein 6
• UniProt Entry Name: TSG6_HUMAN

NCBI Description

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]

Uniprot Description

TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: extracellular space

Molecular Function: hyaluronic acid binding

Biological Process: ovulation; cell-cell signaling; inflammatory response; cell adhesion; signal transduction; positive regulation of cell migration