MERS-CoV Spike S2 Subunit Recombinant Protein | MERS-CoV recombinant protein

Recombinant MERS-CoV Spike S2 Subunit protein

Cat. Num. AAA201294

• Applications: SDS-Page
• Purity: >85% by SDS-PAGE
• Synonyms: MERS-CoV Spike S2 Subunit; Recombinant MERS-CoV Spike S2 Subunit protein; Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERS-CoV S2 P; Spike2 glycoprotein; S2 glycoprotein; S2; Spike S2 Subunit protein; S2 Subunit; MERS-CoV recombinant protein

• Host: Baculovirus
• Purity/Purification: >85% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 752-1296aa
• Sequence: SVPGEMR LASIAFNHPI QVDQLNSSYF KLSIPTNFSF GVTQEYIQTT IQKVTVDCKQ YVCNGFQKCE QLLREYGQFC SKINQALHGA NLRQDDSVRN LFASVKSSQS SPIIPGFGGD FNLTLLEPVS ISTGSRSARS AIEDLLFDKV TIADPGYMQG YDDCMQQGPA SARDLICAQY VAGYKVLPPL MDVNMEAAYT SSLLGSIAGV GWTAGLSSFA AIPFAQSIFY RLNGVGITQQ VLSENQKLIA NKFNQALGAM QTGFTTTNEA FQKVQDAVNN NAQALSKLAS ELSNTFGAIS ASIGDIIQRL DVLEQDAQID RLINGRLTTL NAFVAQQLVR SESAALSAQL AKDKVNECVK AQSKRSGFCG QGTHIVSFVV NAPNGLYFMH VGYYPSNHIE VVSAYGLCDA ANPTNCIAPV NGYFIKTNNT RIVDEWSYTG SSFYAPEPIT SLNTKYVAPQ VTYQNISTNL PPPLLGNSTG IDFQDELDEF FKNVSTSIPN FGSLTQINTT LLDLTYEMLS LQQVVKALNE SYIDLKELGN YTYYNKWP

• Applicable Applications for MERS-CoV recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: MERS-CoV
• Tag: His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for MERS-CoV recombinant protein

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5% based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. Recombinant MERS-CoV Spike S2 Subunit, fused to His-tag at C-terminus, was expressed in insect cell, and purified by using conventional chromatography techniques.

Product Categories/Family for MERS-CoV recombinant protein

Virus; Proteins

References

Junghyun Goo., et al. (2020) Virus Res. 278:197863. Yan-Hua Li., et al. (2019) Engineering. 5:940-947. Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202. Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.
Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. 2020 Mar;278:197863.{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114870/}

NCBI and Uniprot Product Information

• UniProt Accession #: K0BRG7
• Molecular Weight: 60.7kDa(554aa)