Guinea Pig Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit | ENA-78 elisa kit

Guinea pig Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit

Cat. Num. AAA263352

• Gene Names: CXCL5; SCYB5; ENA-78
• Reactivity: Guinea Pig
• Synonyms: Epithelial neutrophil activating peptide 78 (ENA-78); Guinea pig Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit; ENA-78 elisa kit

• Reactivity: Guinea Pig
• Sequence Length: 114

• Samples: Guinea pig serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ENA-78 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Guinea pig ENA-78 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 471243
• NCBI GeneID: 6374
• UniProt Accession #: P42830; Q96QE1
• Molecular Weight: 11,972 Da
• NCBI Official Full Name: ENA-78
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: CXCL5
• NCBI Official Synonym Symbols: SCYB5; ENA-78
• NCBI Protein Information: C-X-C motif chemokine 5; neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; epithelial-derived neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: CXCL5
• UniProt Synonym Gene Names: ENA78; SCYB5
• UniProt Entry Name: CXCL5_HUMAN

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

Uniprot Description

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; chemotaxis; signal transduction