Chicken ENA-78 ELISA Kit | ENA-78 elisa kit

Chicken ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit

Cat. Num. AAA2503256

• Gene Names: CXCL5; SCYB5; ENA-78
• Reactivity: Chicken
• Synonyms: ENA-78; Chicken ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit; ENA-78 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken ENA-78. No significant cross-reactivity or interference between Chicken ENA-78 and analogues was observed.
• Sequence Length: 114

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ENA-78 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken ENA-78 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ENA-78. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ENA-78 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ENA-78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ENA-78. You can calculate the concentration of ENA-78 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 471243
• NCBI GeneID: 6374
• UniProt Accession #: P42830; Q96QE1
• Molecular Weight: 11,972 Da
• NCBI Official Full Name: ENA-78
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: CXCL5
• NCBI Official Synonym Symbols: SCYB5; ENA-78
• NCBI Protein Information: C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: CXCL5
• UniProt Synonym Gene Names: ENA78; SCYB5
• UniProt Entry Name: CXCL5_HUMAN

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

Uniprot Description

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; chemotaxis; signal transduction; inflammatory response