Western Blot (WB)
(Figure 1. Western blot analysis of Lgi1/EPT/LGI1 using anti-Lgi1/EPT/LGI1 antibody (MBS1753376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse Neuro-2a whole cell lysatesLane 4: human HEK293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Lgi1/EPT/LGI1 antigen affinity purified polyclonal antibody (Catalog # MBS1753376) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Lgi1/EPT/LGI1 at approximately 64KD. The expected band size for Lgi1/EPT/LGI1 is at 64KD. )
Rabbit Lgi1/EPT/LGI1 Polyclonal Antibody | anti-LGI1 antibody
Anti-Lgi1/EPT/LGI1 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Lgi1/EPT/LGI1 using anti-Lgi1/EPT/LGI1 antibody (MBS1753376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse Neuro-2a whole cell lysatesLane 4: human HEK293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Lgi1/EPT/LGI1 antigen affinity purified polyclonal antibody (Catalog # MBS1753376) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Lgi1/EPT/LGI1 at approximately 64KD. The expected band size for Lgi1/EPT/LGI1 is at 64KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Lgi1/EPT/LGI1 using anti-Lgi1/EPT/LGI1 antibody (MBS1753376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse Neuro-2a whole cell lysatesLane 4: human HEK293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Lgi1/EPT/LGI1 antigen affinity purified polyclonal antibody (Catalog # MBS1753376) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Lgi1/EPT/LGI1 at approximately 64KD. The expected band size for Lgi1/EPT/LGI1 is at 64KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-Lgi1/EPT/LGI1 antibody (MBS1753376).Overlay histogram showing U20S cells stained with MBS1753376 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lgi1/EPT/LGI1 Antibody (MBS1753376, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-Lgi1/EPT/LGI1 antibody (MBS1753376).Overlay histogram showing U20S cells stained with MBS1753376 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lgi1/EPT/LGI1 Antibody (MBS1753376, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Bigner, S. H., Vogelstein, B. Cytogenetics and molecular genetics of malignant gliomas and medulloblastoma. Brain Path. 1: 12-18, 1990.
3. Bisulli, F., Tinuper, P., Scudellaro, E., Naldi, I., Bagattin, A., Avoni, P., Michelucci, R., Nobile, C. A de novo LGI1 mutation in sporadic partial epilepsy with auditory features. Ann. Neurol. 56: 455-456, 2004.
NCBI and Uniprot Product Information
NCBI Description
This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. [provided by RefSeq, Jul 2008]
Uniprot Description
LGI1: Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. Defects in LGI1 are the cause of epilepsy, familial temporal lobe, type 1 (ETL1). A focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: extracellular space; extracellular region; synapse; cell junction
Molecular Function: protein binding; receptor binding
Biological Process: cell proliferation; nervous system development; axon guidance; positive regulation of cell growth; neurite development; positive regulation of synaptic transmission; protein homooligomerization
Disease: Epilepsy, Familial Temporal Lobe, 1
Research Articles on LGI1
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Product Notes
The LGI1 lgi1 (Catalog #AAA1753376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lgi1/EPT/LGI1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lgi1/EPT/LGI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the LGI1 lgi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lgi1/EPT/LGI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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