Western Blot (WB)
(Mouse Gsk3b Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow).)
Rabbit anti-Mouse Gsk3b Polyclonal Antibody | anti-Gsk3b antibody
Gsk3b, NT (Gsk3b, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B) (PE)
Gene Names
Gsk3b; GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Gsk3b; Polyclonal Antibody; NT (Gsk3b; Glycogen synthase kinase-3 beta; Serine/threonine-protein kinase GSK3B) (PE); Serine/threonine-protein kinase GSK3B; anti-Gsk3b antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-Gsk3b antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Gsk3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of Mouse Gsk3b.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Gsk3b Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow).)
Western Blot (WB)
(Mouse Gsk3b Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Gsk3b Antibody (N-term) (AP14847a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Gsk3b Antibody (N-term) (AP14847a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Gsk3b antibody
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity).
Product Categories/Family for anti-Gsk3b antibody
References
Liu, Y., et al. Diabetologia 53(12):2600-2610(2010). Graham, J.A., et al. J. Biol. Chem. 285(43):32852-32859(2010). Ahmad, I., et al. Oncogene (2010). In press: Asada, N., et al. J. Neurosci. 30(26):8852-8865(2010). Carew, R.M., et al. BMC Dev. Biol. 10, 73 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,710 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 1
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
Gsk3b
NCBI Official Synonym Symbols
GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
NCBI Protein Information
glycogen synthase kinase-3 beta
Protein Family
Research Articles on Gsk3b
Similar Products
Product Notes
The Gsk3b (Catalog #AAA6304678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gsk3b, NT (Gsk3b, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B) (PE) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Gsk3b can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gsk3b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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