Canine Glycogen Synthase Kinase 3 Alpha ELISA Kit | GSK3alpha elisa kit

Canine GSK3alpha (Glycogen Synthase Kinase 3 Alpha) ELISA Kit

Cat. Num. AAA2533651

• Reactivity: Canine
• Synonyms: Glycogen Synthase Kinase 3 Alpha; Canine GSK3alpha (Glycogen Synthase Kinase 3 Alpha) ELISA Kit; GSK3alpha elisa kit

• Reactivity: Canine
• Specificity: This kit recognizes natural and recombinantCanineGSK3alpha. No significant cross-reactivity or interference between CanineGSK3alpha and analogues was observed.
• Sequence Length: 483

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSK3alpha elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of CanineGSK3alphaconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GSK3alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSK3alphaand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSK3alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GSK3alpha.You can calculate the concentration of GSK3alphain the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30354008
• NCBI GeneID: 2931
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: Glycogen synthase kinase 3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.26; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol; mitochondrion

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: aging; cellular protein metabolic process; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glycogen metabolic process; innate immune response; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; positive regulation of neuron apoptosis; positive regulation of protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure; unfolded protein response; Wnt receptor signaling pathway