Rabbit anti-Human ACK1 Polyclonal Antibody | anti-ACK1 antibody

ACK1, NT (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2) (MaxLight 405)

Cat. Num. AAA6461056

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACK1; Polyclonal Antibody; NT (Tyrosine Kinase Non-receptor Protein 2; Activated CDC42 Kinase 1; ACK-1; TNK2) (MaxLight 405); anti-ACK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACK1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 1038

• Applicable Applications for anti-ACK1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; FLISA: 1:1000; IHC: 1:50-1:100; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within residues 17-47 in the N-terminal region of human ACK1.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACK1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using ACK1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ACK1 antibody

Antibodies; Protein Kinases

References

1. Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003). 2. Manser, E., et al., Nature 363(6427):364-367 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 56549666
• NCBI GeneID: 10188
• NCBI Accession #: NP_005772
• NCBI GenBank Nucleotide #: NP_005772.3
• UniProt Accession #: Q07912
• Molecular Weight: 114.327
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase non receptor 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activator of C kinase protein
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.11.1; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extrinsic to internal side of plasma membrane; adherens junction; growth cone; cytoplasmic vesicle membrane; cell soma; membrane; clathrin-coated vesicle; axon; cytoplasm; dendrite; plasma membrane; coated pit; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; WW domain binding; ATP binding; GTPase inhibitor activity; hormone receptor binding; epidermal growth factor receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation