Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ULK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Rabbit anti-Human ULk1 Polyclonal Antibody | anti-ULk1 antibody
ULk1 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ULK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ULK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Immunofluorescence (IF)
(Immunofluorescence analysis using ULk1 antibody.Green: ULK-1 protein stained by anti-ULK-1 antibody diluted at 1:300. Red: alpha-tubulin stained by monoclonal anti-tubulin antibody diluted at 1:100. Scale bar = 10 um.)
Immunofluorescence (IF)
(Immunofluorescence analysis using ULk1 antibody.Green: ULK-1 protein stained by anti-ULK-1 antibody diluted at 1:300. Red: alpha-tubulin stained by monoclonal anti-tubulin antibody diluted at 1:100. Scale bar = 10 um.)
NCBI and Uniprot Product Information
Molecular Weight: 1050
Uniprot Description
ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Autophagy; Protein kinase, Other; EC 2.7.11.1; Other group; ULK family
Chromosomal Location of Human Ortholog: 12q24.3
Cellular Component: autophagic vacuole; cytoplasm; cytosol; pre-autophagosomal structure membrane
Molecular Function: ATP binding; protein binding; protein complex binding; protein serine/threonine kinase activity; Rab GTPase binding
Biological Process: axon extension; cellular response to nutrient levels; macroautophagy; negative regulation of protein complex assembly; neurite development; neurite regeneration; peptidyl-serine phosphorylation; positive regulation of autophagy; positive regulation of macroautophagy; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein localization; regulation of autophagy; response to starvation; signal transduction
Research Articles on ULk1
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Product Notes
The ULk1 ulk1 (Catalog #AAA9129951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ULk1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ULk1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:1000, IF: 1:10 - 1:100. Researchers should empirically determine the suitability of the ULk1 ulk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULk1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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