SDS-Page
(Recombinant GSG2 (470-798) / HASPIN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 38.7 kDa Purity: >80%)
GSG2 recombinant protein
Recombinant GSG2 (470-798) protein
Gene Names
GSG2; HASPIN
Synonyms
GSG2; Recombinant GSG2 (470-798) protein; Histone H3 Associated Protein Kinase; GSG2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant GSG2 (470-798)/HASPIN protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant GSG2 (470-798)/HASPIN protein that includes amino acids 470-798 of human GSG2 protein (accession number NP_114171.2) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant GSG2 (470-798) / HASPIN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 38.7 kDa Purity: >80%)
SDS-Page
(Recombinant GSG2 (470-798) / HASPIN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 38.7 kDa Purity: >80%)
Testing Data
(HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for GSG2 (470-798) / HASPIN activity 1 uM STK S1 substrate was incubated with different concentrations of GSG2 (470-798) / HASPIN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for GSG2 recombinant protein
Background: HASPIN (Histone H3 Associated Protein Kinase) is a serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. It may act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
Product Categories/Family for GSG2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,495 Da
NCBI Official Full Name
serine/threonine-protein kinase haspin
NCBI Official Synonym Full Names
germ cell associated 2 (haspin)
NCBI Official Symbol
GSG2
NCBI Official Synonym Symbols
HASPIN
NCBI Protein Information
serine/threonine-protein kinase haspin; H-haspin; germ cell-specific gene 2 protein; haploid germ cell-specific nuclear protein kinase
UniProt Protein Name
Serine/threonine-protein kinase haspin
Protein Family
UniProt Gene Name
GSG2
UniProt Entry Name
HASP_HUMAN
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Product Notes
The GSG2 gsg2 (Catalog #AAA389418) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GSG2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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