A33 Recombinant Protein | GPA33 recombinant protein

A33 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122821

• Gene Names: Gpa33; BB116197; 2010310L10Rik; 2210401D16Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: A33; A33 Protein; Mouse; Recombinant (His Tag); Mouse GPA33/Glycoprotein A33 Protein (His Tag); 2010310L10Rik Protein; 2210401D16Rik Protein; BB116197 Protein; glycoprotein A33 (transmembrane); GPA33 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile235

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse GPA33 (NP_067623.1) extracellular domain (Met 1-Ile 235) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GPA33 recombinant protein

Background: Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

Product Categories/Family for GPA33 recombinant protein

Cancer Drug Targets

References

Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74. Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6. Frey,D. et al., 2008, Cancer Biother Radiopharm 23 (1):65-73. Rageul,J. et al., 2009, Int J Cancer 125 (12):2802-9.

NCBI and Uniprot Product Information

• NCBI GI #: 11037800
• NCBI GeneID: 59290
• NCBI Accession #: NP_067623.1
• NCBI GenBank Nucleotide #: NM_021610.1
• UniProt Accession #: Q9JKA5; Q922D5
• Molecular Weight: 35,692 Da
• NCBI Official Full Name: cell surface A33 antigen
• NCBI Official Synonym Full Names: glycoprotein A33 (transmembrane)
• NCBI Official Symbol: Gpa33
• NCBI Official Synonym Symbols: BB116197; 2010310L10Rik; 2210401D16Rik
• NCBI Protein Information: cell surface A33 antigen
• UniProt Protein Name: Cell surface A33 antigen
• Protein Family: Cell surface A33 antigen
• UniProt Gene Name: Gpa33
• UniProt Synonym Gene Names: mA33

Uniprot Description

May play a role in cell-cell recognition and signaling.