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Immunohistochemistry (IHC) (Immunochemical staining of human GPA33 in human small intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Rabbit anti-Human A33 Monoclonal Antibody | anti-GPA33 antibody

Recombinant Anti-A33 Antibody, Rabbit Monoclonal

Gene Names
GPA33; A33
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
A33; Monoclonal Antibody; Recombinant Anti-A33 Antibody; Rabbit Monoclonal; GPA33 Antibody; Rabbit MAb; glycoprotein A33 (transmembrane); Anti-A33 Antibody; anti-GPA33 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
071
Specificity
Human GPA33.
No cross-reactivity in ELISA with Mouse GPA33
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GPA33 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:100-1:500
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human GPA33 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human GPA33 (rh GPA33).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GPA33 in human small intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Immunohistochemistry (IHC) (Immunochemical staining of human GPA33 in human small intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Immunohistochemistry (IHC)

(Immunochemical staining of human GPA33 in human colon with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Immunohistochemistry (IHC) (Immunochemical staining of human GPA33 in human colon with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Immunohistochemistry (IHC)

(Immunochemical staining of human GPA33 in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)

Immunohistochemistry (IHC) (Immunochemical staining of human GPA33 in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Related Product Information for anti-GPA33 antibody
Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.
Product Categories/Family for anti-GPA33 antibody
References
Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74. Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6. Frey, D. et al., 2008, Cancer Biother Radiopharm 23 (1):65-73. Rageul, J. et al., 2009, Int J Cancer 125 (12):2802-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
319
NCBI Official Full Name
Cell surface A33 antigen
NCBI Official Synonym Full Names
glycoprotein A33 (transmembrane)
NCBI Official Symbol
GPA33
NCBI Official Synonym Symbols
A33
NCBI Protein Information
cell surface A33 antigen; transmembrane glycoprotein A33
UniProt Protein Name
Cell surface A33 antigen
Protein Family
UniProt Gene Name
GPA33
UniProt Entry Name
GPA33_HUMAN

NCBI Description

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

GPA33: May play a role in cell-cell recognition and signaling.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q24.1

Cellular Component: integral to plasma membrane

Molecular Function: receptor activity

Research Articles on GPA33

Similar Products

Product Notes

The GPA33 gpa33 (Catalog #AAA8105201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-A33 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's A33 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the GPA33 gpa33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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