Human Erythrocyte Membrane Protein Band 4.2 (EPB42) ELISA Kit | EPB42 elisa kit

Human Erythrocyte Membrane Protein Band 4.2 (EPB42) ELISA Kit

Cat. Num. AAA2024731

• Gene Names: EPB42; PA; SPH5
• Reactivity: Human
• Synonyms: Erythrocyte Membrane Protein Band 4.2 (EPB42); Human Erythrocyte Membrane Protein Band 4.2 (EPB42) ELISA Kit; PA; Erythrocyte Surface Protein Band 4.2; EPB42 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Erythrocyte Membrane Protein Band 4.2 (EPB42).; No significant cross-reactivity or interference between Erythrocyte Membrane Protein Band 4.2 (EPB42) and analogues was observed.
• Sequence Length: 691

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythrocyte Membrane Protein Band 4.2 (EPB42) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythrocyte Membrane Protein Band 4.2 (EPB42) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPB42 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythrocyte Membrane Protein Band 4.2 (EPB42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythrocyte Membrane Protein Band 4.2 (EPB42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EPB42 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 215274164
• NCBI GeneID: 2038
• Molecular Weight: 69,455 Da
• NCBI Official Full Name: Erythrocyte membrane protein band 4.2
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.2
• NCBI Official Symbol: EPB42
• NCBI Official Synonym Symbols: PA; SPH5
• NCBI Protein Information: erythrocyte membrane protein band 4.2
• UniProt Protein Name: Erythrocyte membrane protein band 4.2
• Protein Family: Erythrocyte membrane protein
• UniProt Gene Name: EPB42
• UniProt Synonym Gene Names: E42P; Erythrocyte protein 4.2; P4.2
• UniProt Entry Name: EPB42_HUMAN

NCBI Description

Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Epb4.2: Probably plays an important role in the regulation of erythrocyte shape and mechanical properties. Defects in EPB42 are the cause of spherocytosis type 5 (SPH5); also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: cortical cytoskeleton; cytoskeleton; plasma membrane

Molecular Function: protein binding; structural constituent of cytoskeleton; protein-glutamine gamma-glutamyltransferase activity; ATP binding

Biological Process: spleen development; regulation of cell shape; iron ion homeostasis; erythrocyte maturation; hemoglobin metabolic process; cell morphogenesis; peptide cross-linking

Disease: Spherocytosis, Type 5