Typical Testing Data/Standard Curve (for reference only)
()
Human Dolichyl diphosphooligosaccharide protein glycosyltransferase 48 kDa subunit (DDOST) ELISA Kit | DDOST elisa kit
Human Dolichyl diphosphooligosaccharide protein glycosyltransferase 48 kDa subunit (DDOST) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: DDOST ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DDOST antibody and an DDOST-HRP conjugate. The assay sample and buffer are incubated together with DDOST-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDOST concentration since DDOST from samples and DDOST-HRP conjugate compete for the anti-DDOST antibody binding site. Since the number of sites is limited, as more sites are occupied by DDOST from the sample, fewer sites are left to bind DDOST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDOST concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Uniprot Description
DDOST: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Defects in DDOST are the cause of congenital disorder of glycosylation type 1R (CDG1R). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the DDOST 48 kDa subunit family.
Protein type: Transferase; Endoplasmic reticulum; EC 2.4.99.18; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p36.1
Cellular Component: endoplasmic reticulum membrane; protein complex; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane
Molecular Function: protein binding; dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity
Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; T cell activation; translation; response to cytokine stimulus; protein amino acid N-linked glycosylation; innate immune response; protein amino acid glycosylation; gene expression; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Disease: Congenital Disorder Of Glycosylation, Type Ir
Research Articles on DDOST
Similar Products
Product Notes
The Human DDOST ddost (Catalog #AAA7200685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200685 ELISA Kit recognizes Human DDOST. It is sometimes possible for the material contained within the vial of "Dolichyl diphosphooligosaccharide protein glycosyltransferase 48 kDa subunit (DDOST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
