Nectin 3 Active Protein | NECTIN3 active protein

Nectin 3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120524

• Gene Names: PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Nectin 3; Nectin 3 Protein; Human; Recombinant (His Tag); Human CD113/Nectin-3/PVRL3 Protein (His Tag); CD113 Protein; CDw113 Protein; DKFZp566B0846 Protein; FLJ90624 Protein; nectin-3 Protein; PPR3 Protein; PRR3 Protein; PVRL3 Protein; PVRR3 Protein; poliovirus receptor-related 3; NECTIN3 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp400

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized recombinant human PVRL3 at 1ug/ml (100uL/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800ng/mL.; 2. Immobilized recombinant human PVRL3 at 1ug/ml (100uL/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 58
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human PVRL3 (NP_056295.1)(Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. 1. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml. 2. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.)

Related Product Information for NECTIN3 active protein

Background: Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in the reduction of cell movement and proliferation.

References

Satoh-Horikawa, et al. (2000). Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. J Biol Chem (UNITED STATES). 275 (14): 10291-9.Reymond N, et al. (2000). Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin. Gene (NETHERLANDS). 255 (2): 347-55.

NCBI and Uniprot Product Information

• NCBI GI #: 11386199
• NCBI GeneID: 25945
• NCBI Accession #: NP_056295.1
• NCBI GenBank Nucleotide #: NM_015480.2
• NCBI Official Full Name: nectin-3 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: PVRL3
• NCBI Official Synonym Symbols: PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• NCBI Protein Information: nectin-3; nectin 3; poliovirus receptor-related protein 3
• Protein Family: Nectin

NCBI Description

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]