Mouse MP2K1 Monoclonal Antibody | anti-MAP2K1 antibody

MP2K1 Antibody

Cat. Num. AAA9217036

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Synonyms: MP2K1; Monoclonal Antibody; MP2K1 Antibody; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK1; PRKMK1; anti-MAP2K1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This MP2K1 antibody is generated from a mouse immunized with a recombinant protein between 1-393 amino acids from human MP2K1.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 393

• Applicable Applications for anti-MAP2K1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:4000
• Subtitle: Purified Mouse Monoclonal Antibody (Mab)
• Cellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
• Tissue Location: Widely expressed, with extremely low levels in brain.
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MP2K1 Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: PC-12 whole cell lysateLane 4: COS-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MAP2K1 antibody

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Product Categories/Family for anti-MAP2K1 antibody

Crown Antibodies; Primary Antibodies; Developmental Biology; Signal Transduction

References

Seger R.,et al.J. Biol. Chem. 267:25628-25631 (1992). Zheng C.-F.,et al.J. Biol. Chem. 268:11435-11439 (1993). Zheng C.-F.,et al.EMBO J. 13:1123-1131 (1994). Duesbery N.S.,et al.Science 280:734-737 (1998). Vitale G.,et al.Biochem. J. 352:739-745 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q02750
• Molecular Weight: 43439 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Protein kinase, STE; Kinase, protein; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.1-q22.33

Cellular Component: cytoplasm; cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule organizing center; mitochondrion; nucleus; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein kinase binding; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity; receptor signaling protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; Bergmann glial cell differentiation; cell cycle arrest; cell motility; cell motility involved in cell locomotion; cerebellar cortex formation; chemotaxis; heart development; keratinocyte differentiation; MAPKKK cascade; negative regulation of cell proliferation; neuron differentiation; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; regulation of axon regeneration; regulation of stress-activated MAPK cascade; signal transduction; thymus development; thyroid gland development

Disease: Cardiofaciocutaneous Syndrome 3; Noonan Syndrome 1