Typical Testing Data/Standard Curve (for reference only)
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Goat Thrombospondin 2 (THBS2) ELISA Kit | THBS2 elisa kit
Goat Thrombospondin 2 (THBS2) ELISA Kit
Gene Names
THBS2; TSP2
Reactivity
Goat
Synonyms
Thrombospondin 2 (THBS2); Goat Thrombospondin 2 (THBS2) ELISA Kit; THBS2 elisa kit
Reactivity
Goat
Specificity
This assay has high sensitivity and excellent specificity for detection of THBS2. No significant cross-reactivity or interference between THBS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between THBS2 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
1172
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for THBS2 elisa kit
Intended Uses: This THBS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat THBS2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: THBS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-THBS2 antibody and an THBS2-HRP conjugate. The assay sample and buffer are incubated together with THBS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the THBS2 concentration since THBS2 from samples and THBS2-HRP conjugate compete for the anti-THBS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by THBS2 from the sample, fewer sites are left to bind THBS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The THBS2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: THBS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-THBS2 antibody and an THBS2-HRP conjugate. The assay sample and buffer are incubated together with THBS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the THBS2 concentration since THBS2 from samples and THBS2-HRP conjugate compete for the anti-THBS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by THBS2 from the sample, fewer sites are left to bind THBS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The THBS2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for THBS2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
129,991 Da
NCBI Official Full Name
thrombospondin 2
NCBI Official Synonym Full Names
thrombospondin 2
NCBI Official Symbol
THBS2
NCBI Official Synonym Symbols
TSP2
NCBI Protein Information
thrombospondin-2
UniProt Protein Name
Thrombospondin-2
Protein Family
UniProt Gene Name
THBS2
UniProt Synonym Gene Names
TSP2
NCBI Description
The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]
Uniprot Description
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
Research Articles on THBS2
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Product Notes
The Goat THBS2 thbs2 (Catalog #AAA7260197) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7260197 ELISA Kit recognizes Goat THBS2. It is sometimes possible for the material contained within the vial of "Thrombospondin 2 (THBS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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