Typical Testing Data/Standard Curve (for reference only)
Canine Thrombospondin-2 (THBS2) ELISA Kit | THBS2 elisa kit
Canine Thrombospondin-2 (THBS2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: THBS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-THBS2 antibody and an THBS2-HRP conjugate. The assay sample and buffer are incubated together with THBS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the THBS2 concentration since THBS2 from samples and THBS2-HRP conjugate compete for the anti-THBS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by THBS2 from the sample, fewer sites are left to bind THBS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The THBS2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]
Uniprot Description
THBS2: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disk disease (IDD); also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Belongs to the thrombospondin family.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: extracellular region; basement membrane
Molecular Function: heparin binding; protein binding; calcium ion binding
Biological Process: positive regulation of synaptogenesis; negative regulation of angiogenesis; cell adhesion
Disease: Intervertebral Disc Disease
Research Articles on THBS2
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Product Notes
The Canine THBS2 thbs2 (Catalog #AAA7234429) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234429 ELISA Kit recognizes Canine THBS2. It is sometimes possible for the material contained within the vial of "Thrombospondin-2 (THBS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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